Blar i forfatter "Smaglyukova, Natalia"
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Data on RT-qPCR assay of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) from human uterine endometrial tissue and cancer cells of the uterine cervix
Smaglyukova, Natalia; Sletten, Elise Thoresen; Ørbo, Anne; Sager, Georg (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-06-25)A previous investigation showed that the endometrium normalized in women with endometrial hyperplasia after three months treatment with high dose levonorgestrel IUS (intrauterine system). The effect was maintained even if immunohistochemical analyses of the endometrium showed that nuclear progesterone receptors (nPRs) were completely downregulated. These observations indicated that some type of ... -
Expression of nuclear progesterone receptors (nPR), membrane progesterone receptors (mPR) and progesterone receptor membrane components (PGRMC) in the human endometrium after 6 months levonorgestrel low dose intrauterine therapy
Sletten, Elise Thoresen; Smaglyukova, Natalia; Ørbo, Anne; Sager, Georg (Journal article; Tidsskriftartikkel; Peer reviewed, 2020-05-29)The classical steroid receptors (nuclear receptors), including those for progesterone (nPRs), are thoroughly characterized. The knowledge about so-called non-genomic effects, which are mediated by extra-nuclear initiated signals, has increased immensely the last decades. In a previous clinical study of endometrial hyperplasia, we observed that the antiproliferative progestin effect persisted after ... -
Inhibition of ABCC5-mediated cGMP transport by progesterone, testosterone and their analogues
Odland, Sondre Ulstein; Ravna, Aina Westrheim; Smaglyukova, Natalia; Dietrichs, Erik Sveberg; Sager, Georg (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-13)The biodynamics and biokinetics of sex hormones are complex. In addition to the classical steroid receptors (nuclear receptors), these hormones act through several non-genomic mechanisms. Modulation of ABC-transporters by progesterone represents a non-genomic mechanism. In the present study, we employed inside out vesicles from human erythrocytes to characterize high affinity cGMP transport by ABCC5 ... -
Moderate but not severe hypothermia increases intracellular cyclic AMP through preserved production and reduced elimination
Kuzmiszyn, Adrina Kalasho; Selli, Anders Lund; Furuholmen, Markus; Smaglyukova, Natalia; Kondratiev, Timofei; Fuskevåg, Ole Martin; Sager, Georg; Dietrichs, Erik Sveberg (Journal article; Tidsskriftartikkel; Peer reviewed, 2023-01-14)Rewarming from accidental hypothermia could be complicated by acute cardiac dysfunction but providing supportive pharmacotherapy at low core temperatures is challenging. Several pharmacological strategies aim to improve cardiovascular function by increasing cAMP in cardiomyocytes as well as cAMP and cGMP levels in vascular smooth muscle, but it is not clear what effects temperature has on cellular ... -
Pharmacodynamic properties for inhibition of cAMP- and cGMP elimination by pentoxifylline remain unaltered in vitro during hypothermia
Selli, Anders Lund; Kalasho, Adrina; Smaglyukova, Natalia; Kondratiev, Timofei; Fuskevåg, Ole Martin; Sager, Georg; Dietrichs, Erik Sveberg (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-12-15)Background - Rewarming from hypothermia is associated with severe complications, one of which is hypothermia-induced cardiac dysfunction. This condition is characterized by decreased cardiac output accompanied by increased total peripheral resistance. This contributes to mortality rate approaching 40%. Despite this, no pharmacological interventions are recommended for these patients below 30 °C. ... -
The role of OAT2 (SLC22A7) in the cyclic nucleotide biokinetics of human erythrocytes
Sager, Georg; Smaglyukova, Natalia; Fuskevåg, Ole-Martin (Journal article; Tidsskriftartikkel; Peer reviewed, 2017-12-15) -
Treatment of Cardiovascular Dysfunction with PDE3-Inhibitors in Moderate and Severe Hypothermia—Effects on Cellular Elimination of Cyclic Adenosine Monophosphate and Cyclic Guanosine Monophosphate
Kuzmiszyn, Adrina Kalasho; Selli, Anders Lund; Smaglyukova, Natalia; Kondratiev, Timofei; Fuskevåg, Ole Martin; Lysaa, Roy A; Ravna, Aina Westrheim; Tveita, Torkjel; Sager, Georg; Dietrichs, Erik Sveberg (Journal article; Tidsskriftartikkel; Peer reviewed, 2022-07-13)<p><b>Introduction:</b> Rewarming from accidental hypothermia is often complicated by hypothermia-induced cardiovascular dysfunction, which could lead to shock. Current guidelines do not recommend any pharmacological treatment at core temperatures below 30°C, due to lack of knowledge. However, previous in vivo studies have shown promising results when using phosphodiesterase 3 (PDE3) inhibitors, ... -
Treatment of cardiovascular dysfunction with PDE5-inhibitors - temperature dependent effects on transport and metabolism of cAMP and cGMP
Selli, Anders Lund; Kuzmiszyn, Adrina Kalasho; Smaglyukova, Natalia; Kondratiev, Timofei V.; Fuskevåg, Ole Martin; Lyså, Roy Andre; Ravna, Aina Westrheim; Tveita, Torkjel; Sager, Georg; Dietrichs, Erik Sveberg (Journal article; Tidsskriftartikkel; Peer reviewed, 2021-07-30)<i>Introduction</i>: Cardiovascular dysfunction is a potentially lethal complication of hypothermia. Due to a knowledge gap, pharmacological interventions are not recommended at core temperatures below 30°C. Yet, further cooling is induced in surgical procedures and survival of accidental hypothermia is reported after rewarming from below 15°C, advocating a need for evidence-based treatment guidelines. ...